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2 edition of fermentation of xylose and glucose by a thermophilic bacterium, thermoanaerobacter ethanolicus. found in the catalog.

fermentation of xylose and glucose by a thermophilic bacterium, thermoanaerobacter ethanolicus.

Lynda Suzanne.* Lacis

fermentation of xylose and glucose by a thermophilic bacterium, thermoanaerobacter ethanolicus.

by Lynda Suzanne.* Lacis

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Published .
Written in English


The Physical Object
Pagination106 leaves
Number of Pages106
ID Numbers
Open LibraryOL15427973M

  Thermoanaerobacter sp. X was grown anaerobically in defined medium supplemented with either 50 mM glucose, xylose, fructose or cellobiose as the sole carbon source or 50 mM glucose plus 50 mM xylose as dual carbohydrates at 60°C without shaking. X was passaged five times on the substrate of interest in defined medium before inoculation. In this work, the thermophilic anaerobic bacterium Thermoanaerobacter ethanolicus were introduced to enable xylose utilization while still retaining its inherent ability to grow on 6-carbon substrates. Targeted integration of xylAB into C. thermocellum genome realized simultaneous fermentation of xylose with glucose, with.

Thermoanaerobacter ethanolicus is a gram-positive thermophile that produces considerable amounts of ethanol from soluble sugars and polymeric substrates, including starch. Growth on maltose, a product of starch hydrolysis, was associated with the production of a prominent membrane-associated protein that had an apparent molecular weight of 43, and was not detected in cells grown on xylose. The earliest studies on bioethanol by fermentation of simple s ubtrates by thermophilic clostridia focused on Thermoanaerobacterium saccharolyticum, Thermoanaerobacter brockii, and Clostridium.

Many bacteria and fungi excrete enzymes that degrade starch to facilitate the uptake of carbohydrates into the cell. A number of bacteria belonging to the genera Bacillus, Thermoanaerobacter, Thermoanaerobacterium, Clostridium, Micrococcus, Klebsiella, are able to grow on starch using the extracellular enzyme cyclodextrin glycosyl-transferase (CGTase; EC ) for the initial attack on. Thermophilic anaerobic noncellulolytic Thermoanaerobacter species are of great biotechnological importance in cellulosic ethanol production due to their ability to produce high ethanol yields by simultaneous fermentation of hexose and pentose. Understanding the genome structure of these species is critical to improving and implementing these bacteria for possible biotechnological use in.


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Fermentation of xylose and glucose by a thermophilic bacterium, thermoanaerobacter ethanolicus by Lynda Suzanne.* Lacis Download PDF EPUB FB2

Bacteria. Bacterial treatment of DWW was effective only at a COD below 20 g/L. Therefore, the DWW is either diluted or pretreated. However, thermophilic bacteria were found to be effective in treating raw DWW. Thermophilic bacteria grow at a temperature of above 40°C. Abstract.

The performance of Thermoanaerobacter ethanolicus was evaluated in continuous culture with media containing concentrations of xylose (8 to 20 g/liter) greater than those previously reported. The ethanol yield declined from to to g of ethanol per g of xylose consumed when input xylose was increased from 4 to 20 g/ by:   The fermentation of xylose by Thermoanaerobacter ethanolicus ATCC was studied in pH-controlled batch and continuous cultures.

In batch culture, a dependency of growth rate, product yield, and product distribution upon xylose concentration was observed. With 27 mM xylose media, an ethanol yield of mol ethanol/mol xylose (78% of maximum theoretical yield) was Cited by:   Ethanol fermentation characteristics of Thermoanaerobacter ethanolicus V.

Kannan and R. Mutharasan* Drexel University, Philadelphia, PennsylvaniaUSA (Received 15 June ; revised 21 September ) Thermoanaerobacter ethanolicus is an extreme thermophilic non-spore forming ethanol-producing anaerobic by: Abstract.

Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism.

Here we describe the continuous fermentation of glucose, xylose and arabinose from non-detoxified pretreated wheat straw, birch, corn cob, sugar cane bagasse, cardboard, mixed bio waste, oil palm empty fruit bunch and frond, sugar cane syrup and sugar cane molasses using the anaerobic, thermophilic bacterium Thermoanaerobacter Pentocrobe   We report engineering Thermoanaerobacterium fermentation of xylose and glucose by a thermophilic bacterium, a thermophilic anaerobic bacterium that ferments xylan and biomass-derived sugars, to produce ethanol at high yield.

Knockout of genes involved in organic acid formation (acetate kinase, phosphate acetyltransferase, and L-lactate dehydrogenase) resulted in a strain able to produce ethanol as the only detectable organic.

Key enzymes involved in end-product formation were identified in Thermoanaerobacterium saccharolyticum JW/SL-YS, a thermophilic anaerobic bacterium under consideration as a biological catalyst for the conversion of cellulosic biomass to ethanol. Based on enzymatic assays and genome sequence analyses, pathways were identified that would lead to the.

In this work, the thermophilic bacterium Thermoanaerobacter ethanolicus were introduced to enable xylose utilization while still retaining its inherent ability to grow on 6‐carbon substrates. Targeted integration of xylAB into C. thermocellum genome realized simultaneous fermentation of xylose with glucose.

Several fungi and thermophilic bacteria offer long-term promise and should continue to The two thermophilic genera identified to date are Clostridium and Thermoanaerobacter.

Bacteria are generally recognized to provide high rates at the expense of low ethanol yields; wild-type The major metabolic pathways for xylose fermentation are.

A strictly anaerobic, thermophilic bacterium, designated strain YS13, was isolated from a geothermal hot spring. Phylogenetic analysis using the 16S rRNA genes and cpn60 UT genes suggested strain YS13 as a species of cellobiose or xylose as carbon source, YS13 was able to grow over a wide range of temperatures (45–70 °C), and pHs (pH –), with optimum growth.

Xiong et al. () introduced xylA (encoding for xylose isomerase) and xylB from Thermoanaerobacter ethanolicus to cellulolytic bacteria Clostridium thermocellum DSMachieved simultaneous. Both xylA (encoding for xylose isomerase) and xylB (encoding for xylulokinase) genes from the thermophilic anaerobic bacterium Thermoanaerobacter ethanolicus were introduced to enable xylose utilization while still retaining its inherent ability to grow on 6-carbon substrates.

Thermoanaerobacter yonseiensis sp. nov., a novel extremely thermophilic, xylose-utilizing bacterium that grows at up to 85 degrees C. Kim BC(1), Grote R, Lee DW, Antranikian G, Pyun YR. Author information: (1)Department of Biotechnology and Bioproducts Research Center, Yonsei.

Efficient dark fermentation of isolated strains of both glucose and xylose to generate hydrogen is of considerable practical and academic importance. This study utilized a newly isolated, moderately thermophilic bacterium, W16, to produce hydrogen from xylose, glucose, and mixed xylose and glucose.

Fermentation of sugar mixtures and cellulase loading required for cellulose hydrolysis, both matters of considerable applied interest, are examined in Figs. 3 and and4. ALK2 was cultivated in a 1-L fed-batch fermentor with glucose, xylose, galactose, and mannose each present initially at g/liter for a total concentration of 50 g/liter.

Thermophilic Bacteria is a comprehensive volume that describes all major bacterial groups that can grow above °C (excluding the Archaea). Over 60 different species of aerobic and anaerobic thermophilic bacteria are covered. Isolation, growth methods, characterization and identification, ecology, metabolism, and enzymology of thermophilic bacteria are examined in detail, and an 3/5(1).

All bacteria rapidly utilized xylose before the polymers were utilized at a lower rate. With resting cell suspensions of T. ethanolicus and its mutants, the ethanol formation rates were as high as 60 mmol ( g) and 30 mmol ( g) ethanol per L per h from glucose and xylose, respectively.

The genes encoding xylose isomerase (xylA) and xylulose kinase (xylB) from the thermophilic anaerobe Thermoanaerobacter ethanolicus were found to constitute an operon with the transcription initiation site nucleotides upstream from the previously assigned (K.

Dekker, H. Thermoanaerobacter ethanolicus GrowthandProductYield from Elevated Levels ofXylose or Glucose in Continuous Cultures One group of thermophilic bacteria, the anaerobic, sac-charolytic ethanologens, havebeen proposed as a meansof some success has been achieved with the fermentation of xylose.

Thisis significant, since xylose composesupto. @article{osti_, title = {Production of ethanol from biopolymers by anaerobic, thermophilic, and extreme thermophilic bacteria. III. Thermoanaerobacter ethanolicus JW and its mutants in batch cultures and resting cell experiments}, author = {Wiegel, J and Carreira, L H and Mothershed, C P and Puls, J}, abstractNote = {Several thermophilic and extreme thermophilic anaerobic bacteria.

A novel thermophilic Gram staining positive strain Rx1 was isolated from hot springs in Baoshan of Yunnan Province, China. The strain was characterized as a hemicellulose-decomposing obligate anaerobe bacterium that is rod-shaped (diameter: – μm; length: – μm), spore-forming, and motile.

Its growth temperature range is 38–68 °C (optimum 50–55 °C) and pH range is .Thermoanaerobacter ethanolicus is a Gram-positive thermophile that converts xylose to ethanol. A portion of the T. ethanolicus xylose transport permease gene (xylH) was cloned, and the deduced.